Dual-Isotope Cryoimaging Quantitative Autoradiography: Investigating Antibody–Drug Conjugate Distribution and Payload Delivery Through Imaging

Cryoimaging-Microscopy Implementation for 3D Optical Imaging

Quantitative Receptor Autoradiography

This collection of writings reflects the author's unique capacity to choose a topic, to mull over it gracefully, and to evoke the perplexing nature of a number of fundamental notions (and the sentiments which these themes engender) regarding the human experience, i.e., life and death. While the writing style and diction are often in and of themselves as complex and intricate as any textbook des...

Quantitative Electron Microscopic Autoradiography

Quantitative electron microscopic autoradiography was used for comparing the binding of labeled somatostatin-14 (S-14) and somatostatin-28 (S-28) to islet cells. Monolayer cultures of rat islet cells were incubated with ['251-Tyr"jS-14 (S-14) or [125I-Leu8, D-Trp22, TyreJS-28 (S-281) in the presence or absence of excess unlabeled peptides. Autoradiographic grains (ARG) associated with individua...

Quantitative microbial ecology through stable isotope probing.

Bacteria grow and transform elements at different rates, and as yet, quantifying this variation in the environment is difficult. Determining isotope enrichment with fine taxonomic resolution after exposure to isotope tracers could help, but there are few suitable techniques. We propose a modification to stable isotope probing (SIP) that enables the isotopic composition of DNA from individual ba...

A densitometer for quantitative autoradiography.

A low cost spot densitometer system is described. This system is useful for quantitative autoradiography of local cerebral glucose utilization, blood flow, receptor binding and other applications requiring densitometry on films. The densitometer can be used alone or interfaced to a microcomputer. The densitometer consists of a photographic enlarger, a digital multimeter, and the densitometer el...